Yeast transformation - conventional heat shock method, Ito et al
Since i couldn't get any transformant from the previous transformation by electroporation i decided to concentrate on heat shock method. This method was designed for Saccharomyces cerevisiae, therefore the probability for me to get the tansformant might as well as low as the electroporation. So, after doing the procedure for three times i've finally got a single distinct colony with size ~ 2mm for each pKLACNA and pKLAC1-malE (+ve control: encode for maltose binding protein) transformed cell. In order to confirm whether the colony is a positive transformant or not, i'll have to extract the DNA and do the PCR.
Wish me luck!!!
